IMAGE

Fig. S7

ID
ZDB-IMAGE-150401-26
Source
Figures for Zhao et al., 2014
Image
Figure Caption

Fig. S7 Leptin signaling regulates the generation of MG-derived progenitors. Related to Figure 5. (A) qPCR quantification of injury-dependent gene induction in FACS purified GFP+ MG-derived progenitors from the retina of 1016tuba1a:gfp fish at 4 dpi in comparison to that in FACS purified GFP+ MG from the uninjured retina of gfap:gfp fish; *P<0.05, n=3. (B) BrdU immunofluorescence shows that Leptin receptor and Gp130 knockdown inhibits the generation of BrdU+ MG-derived progenitors at 4 dpi. Asterisks mark the injury sites and the arrows point to MG-derived progenitors. (C) Quantification of the effects Gp130 and Leptin receptor knockdown have on progenitor formation (Figure 4C; Figure 5C); ***P<0.001, n=4. (D) qPCR showing Gp130 and Leptin receptor knockdown, individually or in combination, inhibits injury-dependent induction of reprogramming genes at 2 dpi; **P<0.01, ***P<0.001, n=4. (E) Knockdown of Gp130 inhibits the expression of Stat3-GFP at 4 dpi. (F) Quantification of the number of GFP+ cells in the retina at 4 dpi following Gp130 or Leptin knockdown; ***P<0.001, n=4. Error bars, s.d. (G) Quantification of the number of BrdU+ cells expressing gp130 or lepr RNA in the retina at 4 dpi; n=3. Error bars, s.d. (H) Effects of different Leptin concentrations on the generation of BrdU+ MG-derived progenitors and transgene expression in the uninjured retina of 1016tuba1a:gfp fish. The arrows point to MG-derived progenitors. (I, J) Quantification of the effect different Leptin (I) or Leptin/IL-11 (J) concentrations have on progenitor formation in the uninjured retina; n=3. Error bars, s.d. (K) GFP immunofluorescence using a secondary antibody coupled to a red fluor shows autofluorescence in the green channel (left-hand panel, dot) that is localized to the ONL and pigment epithelium, while the red channel shows GFP expression restricted to the injury site (right-hand panel, asterisk). Scale bars, 20 µm (B); 50 µm (E, H, K). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.