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Fig. 3
Actin dynamics has a pivotal role in chirality of cellular rotation. (A) A rotating melanophore from a Tg(mitfa: Histone H2B-RFP, mitfa:lifeact-GFP, golden) fish. The lower panels show magnified images of those in the upper panels. The numbers in the upper panels indicate the time after the medium was exchanged (h: min). Scale bars represent 20 µm. White arrowheads indicate a rotating nucleus (magenta). The actin fibers were visualized with Lifeact-GFP (green). White double-headed arrows indicate bundles of actin fibers, which rotated clockwise. See Movie S6 (Supporting Information). (B) A model of cellular rotation of melanophore. Red arrows represented the direction of cellular rotation. Note that the direction is counterclockwise from basement view. Green arrows represented the direction of growth of actin fibers. Blue arrows represented the direction of the movement of actin fibers. The newly synthesized actin fibers pushed apical cell body in anticlockwise direction.