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Fig. 4
Gpr22 is not involved in KV formation or morphology.
(A) WISH for the DFC marker casanova on embryos at 75% epiboly or (B) for the KV marker charon on 10 somite stage embryos. Shown is (A) the dorsal view (B) of the tailbud region with anterior to the bottom. gpr22 overexpression or knock down does not alter the expression of casanova or charon. MO = morpholino, KV = Kupffer’s vesicle, WISH = whole mount in situ hybridization, DFCs = dorsal forerunner cells.
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