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Fig. 6
Sox2 controls PhR cell fate independently of BMP.
(A) The transgenic line Tg(aanat2:GFP) marks the PhRs. (B) The transgenic line Tg(BRE:GFP) is as a BMP signaling reporter. (C) In sox2 morphant embryos, the number of PhRs is increased at 24 hpf, when compared to controls (A). (D) The number of BMP-responsive cells at 24 hpf is unaffected when sox2 is knocked down. (E–F) The number of PhRs (E) and BMP-responsive cells (F) is similar in control embryos at 28 hpf. (G–H) The number of PhRs (G) is higher than the number of BMP-responsive cells (H) at 28 hpf, in sox2 morphants. (I) The average number of PhRs (Tg(aanat2:GFP)-positive cells) and BMP-responsive cells (Tg(BRE:GFP)-positive cells) in control and sox2 morphant embryos, at 24 and 28 hpf. Confocal maximum projections, scale bars = 25 μm, error bars represent ± standard error, ** = p-value <0.001 (MWU test). See also Figure S8 and Movie S3.