|
Fig. 2 Identification of lincRNAs Involved in the Maintenance of mESC Self-Renewal and Pluripotency (A) Knockdown of lincRNA transcription in CCE mESCs 4 days after infection. Transcript levels were measured by qRT-PCR and normalized to Actb mRNA levels. Data are the mean ± SD of triplicates. (B) Fluorescence (upper panels) and bright-field (lower panels) micrographs of Oct4-ESCs after infection with a nontargeting control shRNA or shRNAs targeting Oct4 and six lincRNAs. Scale bars, 100 mm. (C and D) Relative expression of Nanog (C) and Oct4 (D) mRNA after knockdown of 20 selected lincRNAs, each with 2 shRNAs (hairpin 1 and hairpin 2). Expression levels were normalized to Actb. Data are the mean ± SD of triplicates. See also Figure S2 and Table S3.