IMAGE

Fig. S4

ID
ZDB-IMAGE-140306-14
Source
Figures for Su et al., 2014
Image
Figure Caption

Fig. S4 Fgf signaling is required for rescue of the MHB program but not URL specification in gbx- ;otxMO embryos; data in support of Fig. 6.
RNA in situ hybridizations at 22 hpf with the genes indicated on the left (A-H), and Bodipy staining at 2 dpf (I-L). Genotypes are indicated at the top. Embryos are mounted in dorsal view with anterior to the left. (A-D) atoh1a in the URL is absent in fgf8MO (B), but is rescued in fgf8MO;otxMO (C) and even more extensively rescued in gbx-;fgf8MO;otxMO (D). The midbrain (III) and hindbrain (IV) ventricles inflate without forming a sharp isthmic constriction when Fgf8 is knocked down. (E-H) fgf8a expression in anterior r1 is lost in fgf8a morphants (F) and is not rescued by Otx knock-down (G,H). (I-L) 2 dpf embryos stained with CellTraceTM Bodipy, imaged live. Doted lines in WT (I) show the cerebellar primordium (CbP). MHB morphogenesis is disrupted (the III and IV ventricles fuse into a single ventricle) in fgf8MO (J), and is not rescued by knock-down of Otx in otxMO (K) or gbx-;otxMO (L) in spite of prior rescue of cerebellar primordium morphogenesis and URL specification.

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Reprinted from Developmental Biology, 386(1), Su, C.Y., Kemp, H.A., and Moens, C.B., Cerebellar development in the absence of Gbx function in zebrafish, 181-90, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.