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Fig. 2 Effect of the dominant negative FGFR on ntl expression during gastrulation. ntl expression at 50-60% epiboly, polar views, (A,B) in situ hybridisations (purple stain), (C) antibody staining (brown nuclei). In the normal embryo (A) ntl expression appears as a ring of staining throughout the germ ring. (B) Embryo injected with dominant negative FGFR RNA, showing gaps (arrowheads) in Ntl expression. (C) Embryo injected with the dominant negative FGFR and with lac-Z RNA to monitor its distribution; gaps in Ntl staining (arrowheads) correspond with the distribution of β-gal activity (blue nuclei).