Fig. 5

Fig. 5 Real-time imaging of temporally regulated translation. ((A)–(G)) Real-time imaging of temporally regulated translation of tgo3′ (A), 1–523 (B), 524–1197 (C), 524–736 (D), 737–949 (E), 949–1197 (F) and tgo^M32 mRNAs (G). The times after MIH stimulation are shown as standardized time T_GVBD. Arrows indicate ReAsH signals detected at the first time after MIH stimulation. Dotted circles indicate the GV. Bars, 100 µm. (H) Translational timings of the reporter mRNAs after MIH stimulation. Error bars indicate mean±s.e.m. (n=3 for 1–523, 524–736 and tgo^M32 n=5 for 949–1197; n=6 for 524–1197; n=7 for tgo3′ and 737–949). Transgenes indicated by B are translated earlier than those indicated by A, with statistically significant difference (P<0.05, Student2s t-test).