Fig. 4

Fig. 4 Vangl2 functions outside FBM neurons to regulate their migration. (AF) Dorsal views of the hindbrain in 48 hpf host embryos with anterior to the top. Both the donor and host embryos are Tg(isl1:GFP). Donor derived cells contain the lineage tracer rhodamine dextran (red), hence the donor-derived motor neurons are yellow, while the host motor neurons are green. Quantification of non-migrated FBM neurons in r4 and migrated FBM neurons in r5 and r6 is shown in the histograms. Different transplant conditions are indicated on the far left, and written as Donor>Host. Vangl2-/- refers to trilobite mutants. Wildtype, donor-derived motor neurons (yellow cells) in r6 of vangl2 morphant (B) and dtr-/-; vangl2 morphant (F) host embryos are not scored as FBM neurons because they do not have anteriorly directed axons exiting in r4, and are likely glossopharyngeal motor neurons. (A–D) depict control experiments. (E) Many vangl2 mutant FBM neurons migrate out of r4 in a vangl2+/+ environment, even in the absence of host wildtype FBM neurons. (F) Conversely, wildtype FBM neurons never migrate out of r4 in a vangl2-deficient environment, even in the absence of host mutant FBM neurons (compare to B).