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Fig. 1
Transgenic fish associated with neurotransmitter properties. All images were taken from fish that were 3.5 dpf. Genotypes of the transgenic fish are shown at the top of each panel. (A-C) Cross-sections of the spinal cord. Green and red cells do not overlap, indicating that each transgenic fish labels discrete cell populations. (D-G) Lateral views of the spinal cord. (D) Glycinergic neurons derived from dbx1b-positive progenitors are labeled by GFP. (E) GABAergic neurons derived from gsx1-positive progenitors are labeled by GFP. In D and E, neurons in which Cre-mediated recombination did not occur continued to express RFP. (F,G) Glutamatergic neurons (F) and glycinergic neurons (G) derived from dbx1b-positive progenitors are labeled by GFP. Only the green channel is shown. Scale bars: 10 μm (A-C); 20 μm (D-G).