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Fig. S3
Double fluorescent ISH shows mosaic colocalization of enhancer:GFP with endogenous foxd3 and tfap2a mRNA expression. (A-F) Double fluorescent ISH for gfp (A) and foxd3 (B) in foxd3E1:GFP-injected embryos (merge in C, lateral view) and for gfp (D) and tfap2a (E) in tfap2aE2:GFP-injected embryos (merge in F, dorsal view) at 2-somites demonstrate mosaic colocalization of enhancer:GFP with the endogenous gene along the NPB (insets in C,F). (G) tfap2a and gfp also colocalize in the most anterior NPB as shown in a lateral magnification from a separate WT embryo. NPB, neural plate border; tb, tailbud.
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