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Fig. S3
PKD1 silencing in zebrafish did not alter cranial angiogenesis. A–C2, 24 hpf (A, B, C) and 48 hpf (A2, B2, C2) tg(fli1:EGFP) zebrafish embryos were analyzed for defects in the primordial hindbrain channel (PHBC, arrows) and central arteries (CA, asterisks) by confocal microscopy. Injection of 500 pg SB-Mo I5 (B, B2) or 500 pg SB-Mo I6 (C, C2) did not reveal vascular defects in the cranial vasculature as compared to 2 ng Co-Mo injected tg(fli1:EGFP) zebrafish embryos (A, A2). Black scale bars: 500 μm, red scale bars: 50 μm.
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