ZFIN Image: Chernyavskaya et al., 2012, Fig. 5

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Figure Caption

Fig. 5 Physiological studies on heart function in β2.1-depleted embryos. A: Average heart rates at 30 and 48 hours postfertilization (hpf) for embryos were measured in beats per min (bpm). Where noted, embryos were exposed to combinations of 20 μM nifedipine (an LTCC antagonist) for 24 hr (from 24 to 48 hpf), 40 μM BayK (an LTCC agonist) for ~30 min (from 47.5 to 48 hpf) or β2.1 morpholino (injected at the 1-4-cell stage). B–D: The volume of the ventricle at diastole (A), ventricular stroke volume (C), and cardiac output (D) were calculated from selected frames of videos recording ventricular contraction. n = 9 embryos per treatment. B, BayK; C, control; MO, β2.1 morpholino MO1; N, nifedipine.

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Acknowledgments

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