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Fig. 1 Anti-inflammatory activity of the methanolic extract of Rhynchosia viscosa.
Anti-inflammatory activity was determined in an acute inflammation assay based on tail transection and treatment with lipopolysaccharides (LPS). A to D, zebrafish larvae are 4 days post-fertilization (dpf) with anterior to the left, scale bar = 10 μm. After tail transection and LPS exposure, stained leukocytes appear as black-brown spots migrating to the injured area in the transected tails. Migrating leukocytes were counted on one side in the tail in the region to the right of the dashed red arc and migration values were expressed as relative leukocyte migration (RLM) (E). A, tail of an uncut larva; B, negative control (DMSO 1%); C, positive control (indomethacin 100 μM) D, crude extract of R. viscosa at 50 μg/mL; E, graph displaying the RLM of 4 dpf larvae (n = 10) subjected to tail transection and incubation with R. viscosa. RLM d0.5 was established as cutoff for anti-inflammatory activity. * p<0.05.