Fig. 2

Fig. 2 Shape Changes of Shh Gradient and Heterogeneity in Spatial Distribution of Responses (A) Time course of notochord formation by shh:gfp+ cells in cross-section. Red shows mem-mCherry (same below). Arrowhead points to GFP+ cells in neural ectoderm/plate. Arrow indicates MFP cell expressing GFP. Scale bar, 10 μm.(B) Cross-section (i and iii) and longitudinal section (ii and iv) of ptch2:kaede expression pattern. Arrowheads point to neighbor cells with different Kaede levels. Asterisks indicate stereotypic cell fates at the indicated locations. Scale bar, 10 μm. See also Figure S1A.<(C and C′) Kaede level spatial distribution through time. Each mark represents a segmented cell with measured position and fluorescence intensity. (C′) is a spatially averaged (±SD) representation of (C). Kaede intensities in the notochord cells were subtracted as background.