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Fig. S5 Candidate gene analysis for tbx1-/- mutants. Whole mount lateral views (A, B, F, G) and head-on views (C–E, H–J) of 48 hpf embryos stained for known genes that affect heart looping. Expression of tbx2a (A, F), tbx3b (B, G), tbx5 (C, H) and tbx20 (D, I) was unaffected between WT siblings and tbx1-/- mutants. (E, J) Dissected hearts from 48 hpf embryos show down-regulated expression of wnt11r in the mutant (J) versus WT (E). The heart boundary is shown by the red dotted line. Arrows point to the AVC. Scale bars: 50 7mu;m.
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