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Fig. S4 Sarcoplasmic reticulum (SR) is correctly formed in skeletal muscle Nfix-null mice. (A-E′′) Investigation of the SR phenotype both in specific skeletal muscle Nfix-null mouse (Nfix-null) fetal E16.5 and in postnatal P3 developmental stages by means of TEM (A-B′), Western blot analyses (C) and immunostaining for RyR (red, D,E). Laminin staining (green, D′,E′); DAPI staining (blue, D′′,E′′).
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