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Fig. 4 Spatial expression patterns of zebrafish cers4b, cers5, and cers6 in gastrula (8 hours postfertilization [hpf]), 6-somite stage (12 hpf), 18-somite stage (18 hpf), 24 hpf, and 48 hpf embryos. A: For cers4b no expression was detected in early stages, whereas at the 48 hpf stage an expression is found in the brain (b), pectoral fins (pf), and myotomes (mt). B: At gastrula and 6-somites stages, cers5 is expressed in the enveloping layer (EVL), and later at the 18 hpf stage, its expression is pronounced in the periderm and eye (e), particularly the dorsal posterior eye margin. At 24 hpf, cers5 is present in the periderm and eye (e), particularly in a spot in the dorsal posterior region. At 48 hpf, expression is pronounced in the brain (b) pectoral fin (pf) and myotomes (mt). C: At 12 hpf cers6 shows a weak expression in the neural plate (np). At 18 hpf and 24 hpf, cers6 is expressed in spinal cord (sc), trigeminal placodes (tp), and in parts of the developing brain (b). At 48 hpf, cers6 expression is most pronounced in brain (b), medulla oblongata (mo), pectoral fin (pf), and myotomes (mt).