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Fig. 3 her1-/- and her7-/- embryos show mild defects in somite boundary formation and disturbed expression of oscillatory genes in the PSM. (A-C) Zebrafish embryos fixed at ~24 hpf and stained with cb1045 ISH probe to show somite boundaries (lateral views). (A) Wild type (siblings of her7-/- mutant); (B) her1-/-; (C) her7-/-. Arrows indicate irregularities of segmentation. (D-U′) Expression patterns of her1, her7 and deltaC in wild type, her1-/- and her7-/- as shown by conventional ISH (D-L′) and FISH (M-U′; optical sections). Embryos are at the 10-somite stage (14 hpf) and flat mounted. Two specimens from each cohort are shown to illustrate the oscillatory behaviour. Note that the ISH phenotype, like the segmentation phenotype (supplementary material Fig. S1), varied in severity from one batch of embryos to another (compare K,K′ with T,T′). A total of 243 embryos were stained and assessed, including at least 12 for each gene/genotype combination.