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Fig. 3 Teratogenic effects of solvents between 24 and 48 hours post-fertilization.
Embryos at 2 dpf (a, b, c, d) treated for the previous 24 hours with a, embryo medium, b, 1% DMF, c, 1% solketal or d, 2% glycerol. Embryos in b, c, and d display no or very slow blood circulation as well as no touch response (all were immotile). Treatment with 1% DMF or 1% solketal also resulted in brain hemorrhaging (red arrows), pericardial edemas, yolk necrosis (black arrows) and shortening along the AP axis. d, Other than the observed blood flow defects, embryos treated with 2% glycerol appeared morphologically normal. All embryos are depicted with anterior to the left, dorsal to the top.