Fig. 6

Fig. 6 Blebbistatin treatment during early KV development stages disrupts LR patterning. (A and B) RNA in situ hybridizations show normal left-sided spaw expression (arrows) at 16 SS in a control embryo treated with DMSO (A) and bilaterally symmetric spaw expression in a blebbistatin treated embryo (B). (C) spaw expression was significantly altered in embryos treated with blebbistatin between 1–8 SS relative to DMSO controls. (D) Anlaysis of spaw expression in DMSO controls and embryos treated with blebbistatin during the stages indicated. n>100 embryos for all treatments. *Significantly different from controls (p<0.05).