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Fig. S7
Dorsoventral patterning is not affected by HEP induction at 30% epiboly. Embryos from crosses of wild-type and HEP+/- transgenic (Tg) fish were exposed to heat shock at 30% epiboly, allowed to develop to the shield stage at a lower temperature, and then examined for the expression of dorsoventral marker genes (HEP+/-HS, right column). For the control, marker expression is shown for wild-type embryos (WT, left column) and for wild-type embryos exposed to heat treatment (WT-HS, middle column). (A-F, P-R) Animal views with dorsal to the right. (G-O) Lateral views with dorsal to the right and anterior to the top. The angles between the two dashed lines show the sizes of dorsoventral marker expression. The numbers of embryos with normal expression and total embryo numbers are shown at the bottom of the respective panels in the right two columns. Essentially all HEP-treated embryos showed normal marker expression, showing that HEP induction at 30% epiboly does not affect dorsoventral patterning. Scale bar, 200 μm.
Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.