ZFIN Image: Khan et al., 2012, Fig. 10

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Figure Caption

Fig. 10

HEP induction at 30% epiboly upregulates wnt11 expression at the blastoderm margin. Embryos from crosses of wild-type and HEP^+/- transgenic (Tg) fish were exposed to heat shock at 30% epiboly and later examined for gene expression. (A, B) Embryos that were normal or abnormal in terms of gene expression at the bud stage (A) or 50% epiboly (B) were subjected to genotyping. The data demonstrated that normal embryos were negative and abnormal embryos were positive for HEP. G, genomic sequences amplified for the positive control; H, HEP-derived sequences showing that the embryos were transgenic; M, size markers (500 and 200 bp). (C) Embryos subjected to heat shock were examined for wnt11 expression (right two columns). About half of the embryos showed extensive upregulation (HEP-HS). (a–c) Animal views. (d–f) Lateral views. Scale bar, 200 μm.

Acknowledgments

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Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.