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Fig. 8
Rescue of the dorsalizing effects of HEP induction at the sphere stage by pou2 overexpression. Embryos from crosses of wild-type (WT) and HEP+/- transgenic (Tg) fish were injected with egfp (second and third column) or pou2 mRNA (low dose, 30 pg/embryo; fourth to sixth column), exposed to heat shock at the sphere stage, and allowed to develop at lower temperature to the shield stage, at which time they were examined for the expression of DV patterning genes. For the control, wild-type embryos (WT) were stained likewise (leftmost column). Embryos expressing egfp showed either normal (second column) or abnormal expression of DV patterning genes (asterisks, third column). The abnormal expression can be ascribed to HEP induction. When pou2 mRNA was injected in advance, the ratios of embryos showing HEP-dependent effects (asterisks) were greatly decreased (rightmost column). ±, +. ++. +++, ++++, see the footnotes for Table 2.
Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.