Fig. 6

Fig. 6

Dorsalization of embryos by HEP induction at the sphere stage. Embryos from crosses of wild-type and HEP^+/- transgenic (Tg) fish were exposed to heat shock at the sphere stage, allowed to develop to the bud stage at a lower temperature, and then examined for the expression of dorsal markers (right two columns). (A–D) Frontal views with dorsal to the top. (E–L, M–P) Dorsoanterior views with anterior to the top. (I2–L2, M2–P2) Dorsoposterior views with anterior to the top. Embryos in the third column showed normal patterns (Sibling-HS), whereas those in the rightmost column showed abnormal expression (HEP-HS). The number of embryos with abnormal expression and total embryo number are shown at the bottom of the respective panels in the HEP-HS column. For the control, gene expression was examined in parallel in wild-type embryos that were untreated (left column, Wild) or treated with heat shock (second column, Wild-HS). Scale bar, 200 μm.