ZFIN Image: Khan et al., 2012, Fig. 3

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Figure Caption

Fig. 3

Expression of axial and neural genes in embryos injected with high-dose mRNA for pou2-related genes. The expression of axial and neural genes, shown on the left, was examined at the bud stage in embryos injected with high-dose mRNA for egfp (A, F, K, P), pou2 (B, G, L, Q), vp-pou2 (C, H, M, R), mouse Oct-3/4 (D, I, N, S), and en-pou2 (E, J, O, T). The numbers of embryos with abnormal expression patterns and total numbers of embryos examined are shown at the bottom of the respective panels. (F–J, P–T) Dashed lines represent the animal-vegetal axes, showing that pou2-related genes affected anterior extension of the embryo. (K–O) Pairs of dashed lines show lateral edges of the neural plate, revealing that convergence of the neural plate is affected by overexpression of pou2-related genes. Scale bar, 200 μm.

Acknowledgments

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Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.