ZFIN Image: Khan et al., 2012, Fig. 2

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Figure Caption

Fig. 2

Morphological effects of the overexpression of pou2 and its related genes by mRNA injection. mRNA at a low dose (30 pg/embryo, A–E, K–O) and a high dose (90 pg/embryo, F–J, P–T) for egfp (A, F, K, P), pou2 (B, G, L, Q), vp-pou2 (C, H, M, R), mouse Oct-3/4 (D, I, N, S), and en-pou2 (E, J, O, T) was injected into embryos, which were observed at the bud stage (A–J) and 28 hpf (K–T). Lateral views with anterior to the top and dorsal to the right (A–J) or with anterior to the left and dorsal to the top (K–T). (A–J) Dashed lines show animal–vegetal axes, emphasizing incomplete anterior migration of the neural plate (F–J), or connect the tail tips and the centers of the yolk balls, pointing to extension defects of the body axes, in embryos overexpressing a high dose of all four pou2-related genes. icm, intermediate cell mass. Scale bar, 200 μm.

Acknowledgments

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Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.