ZFIN Image: Watkins et al., 2012, Fig. 1

Image

Figure Caption

Fig. 1 Intravital imaging of vascular dynamics in a 5 dpf zebrafish larva.

An extended depth of focus projection (dorsal upwards, anterior left) of the vasculature of a Tg(kdrl:GFP)^la116;Tg(gata1:dsRed)^sd2 embryo with the fluorescence defining the endothelium (A). The outlined box shows the area of the aorta used for the time-based imaging of blood flow velocity and vessel diameter. The lower panel (B) shows a representative image from the same fish of the DsRed labeled erythrocytes within the dorsal aorta. This image was collected with a 2 ms exposure. With these settings it was readily possible to visualize the red blood cells flowing through the dorsal aorta over time. (Scale Bar = 100 microns).

Acknowledgments

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