IMAGE

Fig. 2

ID

ZDB-IMAGE-120813-2

Antibodies

Ab1-aldh2

Source

Figures for Zhou et al., 2012

Image

Figure Caption

Fig. 2

5-Nitrofurans Bind Aldh2 in Zebrafish (A) Biotinylated probes linked to a 5-nitrofuran (Pr-NFN) and a control furan (Pr-FN). Biotin is labeled in blue and the 5-nitro or modification moiety in red. (B) Silver stain of protein bands identified using Pr-NFN probe, or streptavidin beads alone as a control (No Probe). The red box indicates the region of the gel that was isolated for mass spectrometry analysis (arrow) at 57 kD. (C) Western blot of zebrafish protein bound to the no-probe control, the furan (Pr-FN) control, or the 5-nitrofuran probe (Pr-NFN), and probed with zebrafish anti-Aldh2 antibodies. A band corresponding to 57 kDa is indicated (arrow). MW, molecular weight. See also Figure S2 and Table S1.

Figure Data

Expression / Labeling details

Acknowledgments

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