ZFIN Image: Cibrián Uhalte et al., 2012, Fig. 4

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Figure Caption

Fig. 4

Thiophosphorylation of substrate proteins by Prkci^I316 in the zebrafish embryo.

(A) Schematic diagram of the in vivo labeling method for the selective labeling of Prkci^I316A substrates during zebrafish development. (B) In vivo thiophosphorylation in zebrafish embryos injected at the one-cell stage with 200 μM N⁶-benzyl-ATPγS (6-bn-ATPγS) and mRNA encoding either Prkci^WT or Prkci^I316A (AS). Western blot analysis with rabbit monoclonal anti-thiophosphoester (α-thioP) C51-8 antibody (Epitomics) of 80% epiboly (6–8hpf) samples alkylated with 2.5 mM PNBM reveals a selectively labeled protein in the Prkci^I316A (AS) sample (asterisk).

Acknowledgments

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