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Fig. S6 Bmp/Smad signaling antagonists inhibit the ginger-induced bmp7a expression in the area of the developing CHT. Whole-mount in situ hybridization of bmp7a in zebrafish embryos, after treatment with Bmp inhibitors and/or ginger/10-G from 10 to 48 hpf. (A) A control embryo. (B) Zebrafish embryos treated with ginger (5 μg/ml). (C–D) Ginger (5 μg/ml) and dorsomorphin/DMP, 0.1 and 2 μM. (E–G) Ginger (5 μg/ml) and LDN193189, 0.1, 0.5 and 1 μM. (H) Ginger (5 μg/ml) and DMH1, 0.1 μM. (I) Analyses of bmp7a expression localized in the CHT region (table). Scale bars = 300 μm.
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