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Fig. 4 Ginger/10-G treatment after gastrulation promotes bmp2b/7a in the developing caudal hematopoietic tissue.
(A–B) Zebrafish embryos were treated with ginger (5 µg/ml) or 10-G (2 μg/ml) from 10 to 48 hpf, followed by whole-mount in situ hybridization of bmp2b (A) and bmp7a (B). Both bmp2b and bmp7a were up-regulated locally in the CHT (and underlying fin) upon ginger or 10-G exposure (whereas they are not expressed in the CHT of control embryos at 48 hpf). Scale bars = 700 μm.
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