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Fig. 1
The dorsalizing activity of lithium treatment during zebrafish early development.
(A) A severely dorsalized embryo (radialized). (B) A mildly dorsalized embryo. (C) A normal embryo. (D) Diagram demonstrating the dynamics of the dorsalizing capability of acute lithium treatment (0.3 M LiCl for 8 min). The abscissa axis designates the time at which lithium treatment began. The ordinate axis designates the percentage of three kinds of embryos with different degrees of dorsalization at 12.5 hpf. SW1: Sensitive Window 1; SW2: Sensitive Window 2; UW: Unresponsive Window. The data were obtained in three or more separate experiments, and the number of the embryos used for each data set is more than 100. (E) The dorsalizing effect of the lithium treatment is not caused by osmotic stress by comparing with NaCl treatment at the same salt concentration and treatment time. Embryos in A, B, C and E was at 12.5 hpf, and lateral viewed. The bar in A represents 500 μm.