Fig. S9

Fig. S9

Osteoblasts dedifferentiate as they enter the blastema. (A–D, H–K) Sections from two Tg(sp7:EGFP) animals 2 dpa imaged for EGFP expression (A, H, green) and stained with zns-5 antibodies (B, I, red). Nuclei are stained with Hoechst (C, J, blue) and the overlay is shown (D, K). A heavy dashed yellow line indicates the amputation plane and a white dashed line marks the epidermis–blastema boundary. White arrows point to zns-5⁺/EGFP immature, dedifferentiated osteoblasts and the yellow arrows indicate zns-5⁺/EGFP⁺ mature, differentiated osteoblasts. Asterisks mark background-staining red blood cells. (E–G, L–N) Fluorescent signal intensities for EGFP (E, L), zns-5 staining (F, M) and Hoechst (G, N) within the indicated rectangle (thin, dashed yellow line in A–C and H–J) are plotted as 3-dimensional surfaces using ImageJ software. The amputation site is marked with an arrowhead and proximal and distal regions relative to the amputation site are indicated.