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Fig. 3

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ZDB-IMAGE-120329-3
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Figures for Xu et al., 2012
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Fig. 3 Lack of YSL Transcription in nanog-like Morphants Leads to Epiboly and Endoderm Defects
(A) Microarray analysis of gene expression in nanog-like morphants revealed an absence of YSL gene transcription. YSL genes downregulated in three separate experiments are displayed as a heat map.
(B) The expression of gata6, mxtx2, camsap1l1, hnf4a, and slc26a1 is absent in the YSL of nanog-like morphants. All views are lateral with the presumptive dorsal side to the right.
(C) The YSN of the wild-type and nanog-like knockdown embryos at the sphere stage and the 50% epiboly stage labeled with SYTOX green. All views are shown as an animal view with the dorsal side to the right.
(D) Time-lapse microscopy of the YSNs of a nanog-like-MO2-injected embryo revealed the YSN aggregation process. Three YSNs undergo the 12th division at t = 16 min. At t = 22 min, the upper left YSNs separate normally while the lower YSNs fail to completely separate. At t = 36 min, the resulting six daughter YSNs start to aggregate.
(E) Higher magnitude confocal microscopy revealed that the aggregated YSNs in Figure 3D remained separated.
(F) Nanog-like is required autonomously for YSL transcription. To achieve YSL-specific expression, 300 pg nanog-like-Myc mRNA was injected into the yolk at 2.5 hpf. The expression of mxtx2, gata6, and camsap1l1 was rescued by YSL-specific nanog-like-Myc expression.

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Reprinted from Developmental Cell, 22(3), Xu, C., Fan, Z.P., Müller, P., Fogley, R., Dibiase, A., Trompouki, E., Unternaehrer, J., Xiong, F., Torregroza, I., Evans, T., Megason, S.G., Daley, G.Q., Schier, A.F., Young, R.A., and Zon, L.I., Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway, 625-238, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell