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Fig. S1 Efficient TaV2A-Mediated Protein Cleavage in the PhOTO Zebrafish Lines. Western blot analysis of each PhOTO line: lane (1) WT (2) PhOTO-M (3) PhOTO-N. The membrane was probed for Dendra2 as well as α-Tubulin (loading control, upper blot). No Dendra2 was seen in the WT (lane 1), and each PhOTO lane had the Dendra2 protein at approximately the molecular weight expected from the particular fusion protein. Note that for lane 2, two bands are present, reflecting the post-translational palmitoylation/myristoylation additions. Efficient protein cleavage as a result of the TaV 2A sequence was confirmed by the lack of an uncleaved product within the two PhOTO lanes. (absence of a band above 50kDa in the lower blot).