ZFIN Image: Gerhart et al., 2012, Fig. 1

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Figure Caption

Fig. 1

The Cx40.8 antibody is specific.

(A) An antibody raised against a Cx40.8 carboxy-terminal peptide detects a single major band from regenerating fin lysates (arrowhead). Non-competed (B) and competed (C) anti-Cx40.8 antibody was used to detect bacterially expressed GST-Cx40.8CT. Decreasing volumes of a concentrated lysate were loaded in each lane on two identical gels. When the antibody was pre-incubated with the Cx40.8 target sequence (i.e. competed), antibody binding was reduced in all lanes compared with non-competed antibody. Arrowhead points to the full length product. Bands at molecular weights smaller than the full-length fusion protein represent degradation products.

Acknowledgments

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