ZFIN Image: Kuo et al., 2011, Fig. S1

Image

Figure Caption

Fig. S1

The release of soluble THSD7A is protease-dependent. A. THSD7A-transfected HEK293T cells were treated with complete protease inhibitor (C), Benzamidine (B), Leupeptin (L) and Pepstatin A (P). Untreated cells (No) served as a control. Cell lysates and cultured medium were subjected to Western blot with anti-sTHSD7A antibody. GAPDH served as a loading control. B. Two proteases, thrombin and caspase 1, are predicted to cleave the full-length THSD7A. C. THSD7A-transfected HEK293T cells were treated with complete protease inhibitor (C), hirudin (H), caspase 1 inhibitor I (I) or no treatment (No). Cell lysates and cultured medium were subjected to Western blot with anti-sTHSD7A antibody. GAPDH served as a loading control.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One