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Fig. 6
Soluble THSD7A promotes focal adhesion assembly to enhance cell migration via FAK-dependent manner.
A. Adhesion assay performed with HUVEC in the presence and absence of soluble THSD7A on type I collagen- or PLL-coated coverslips. B. Immunostaining of actin filaments (red) and nuclei (blue) in HUVEC. The quantitative data show the average number of filopodia per cell. C. Immunostaining of vinculin (green) and nuclei (blue) in HUVEC. The quantitative data show the average number of peripheral vinculin plaques per cell. D. Immunostaining of p-FAK (green) and nuclei (blue) in HUVEC. The quantitative data show the average number of peripheral p-FAK plaques per cell. Bar represents 10 μm. *P<0.05 vs. vector. E, HUVEC lysates treated with soluble THSD7A or control medium were subjected to Western blot. Relative fold levels of p-FAK, p- Erk1/2, p-P38, p-Akt and GAPDH are represented as the mean ± SD. Total levels of FAK, Erk1/2, P38 and Akt served as loading controls. Each experiment was repeated at least three independent times.