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Fig. 2
THSD7A is a membrane-associated protein.
THSD7A-transfected HEK293T cells were homogenized and the cytosolic (C), nuclear (N) and membrane (M) fractions were prepared as described in the materials and methods. Each fraction was subjected to Western blot with anti-THSD7A (IDS2, IDS9, CTE), anti-FLAG-tag, anti-calnexin (plasma membrane marker), anti-annexin V (cytoplasm and plasma membrane marker) and anti-GAPDH (cytoplasm marker) antibodies. Arrow indicates the carboxyl-terminal fragment of THSD7A.
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