ZFIN Image: Deng et al., 2011, Fig. S4

Image

Figure Caption

Fig. S4

Figure S4. Fate-mapping of neutrophils from CHT, related to Figure 5.

(A) Schematics of photoconversion. UV converts green fluorescent Dendra2 to red, allowing for fate-mapping.

(B) Photolabeled neutrophils responded to the wound in a control morphant, whereas in a Rac2 morphant, neutrophils did not migrate to the wound, but entered circulation. Locations of wounds were highlighted. Arrow, circulating neutrophil. Images are representative for 6 larvae from 2 separate experiments. Scale bar, 50 μm.

(C) Neutrophil in the CHT at 2dpf Rac2 morphants spontaneously entered circulation 18 h after photo-conversion. Still images from one movie representative for 9 zebrasfish (3 independent experiments) are shown. Location of blood vessel is highlighted. Arrow, circulating photoconverted neutrophils. Arrow head, a circulating non-photoconverted neutrophil. Scale bar, 30 μm.

Acknowledgments

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Reprinted from Developmental Cell, 21(4), Deng, Q., Yoo, S.K., Cavnar, P.J., Green, J.M., and Huttenlocher, A., Dual roles for Rac2 in neutrophil motility and active retention in zebrafish hematopoietic tissue, 735-745, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell