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Fig. 3
Behavior and migration of a transplanted eel PGC in zebrafish host embryo.
(A) An eel PGC extended a long filopoduim-like process and moved actively in zebrafish embryos (also see Movie S2). The time elapsed (in minutes) from the time imaging was begun can be seen at the top of each figure. Arrows indicate the filopodium-like process. (B) A transplanted eel PGC migrated along with endogenous zebrafish PGCs toward the area of future gonad formation. The GFP-labeled cell is a transplanted eel PGC and RFP-labeled cells are endogenous zebrafish PGCs (also see Movie S3). Boxed areas with red dashed lines in the upper illustration indicate the region in the corresponding photograph. (C) A transplanted eel PGC that has migrated to the precise region of future gonad formation in the zebrafish embryo. The two smaller images in the middle show the corresponding boxed areas at a higher magnification. The scale bars in A and B represent 10 μm and 50 μm, respectively.