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Fig. S5
Endodermal frizzled5 expression includes the presumptive hepatic domain at the time of liver specification. (A-E2) fzd5 is widely expressed in the endoderm at 20 (A), 22 (B,D-E2) and 26 (C) hpf, including the presumptive hepatic domain (dashed bracket) anterior to the endocrine islet and the more posteriorly located intestine. Insulin-expressing endocrine islet cells (dark, high-level expressing cells; plain bracket) serve as anteroposterior landmark. (D-E2) endodermal fzd5 expression includes the hepatic endoderm, located at the height of somites 1-3 (black line), which are visualized by α-Myosin staining (D2,E2). (A-D2) Dorsal views, (E,E2) lateral view; (A-C) anterior is towards the top; (D-E2) anterior is towards the left.