ZFIN Image: Xiao et al., 2011, Fig. S1

Image

Figure Caption

Fig. S1

RGC Axon Projections Require Slit/Robo Signaling, Related to Figure 2(A–C) Projections of 6 dpf Pou4f3:mGFP WT (A), ast^ti272z (B), and hsp70l:Slit2-GFP fish (C). Heat shock was applied at 76 hpf.(D–F) Optical sections of tectum of 5 dpf Pou4f3:mGFP fish. Arrows point at RGC axons aberrantly crossing midline. Pou4f3:mGFP-labeled RGC axons occupy about 60% of the depth of the tectal neuropil (D). In both ast^ti272z mutant (E) and slit1a MO (F), Pou4f3:mGFP-labeled RGC axons are more dispersed in the tectal neuropil. Yellow lines in D to F indicate the depth of the tectal neuropil. Green lines in D to F indicate the depth of Pou4f3:mGFP expressing RGC axons.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 146(1), Xiao, T., Staub, W., Robles, E., Gosse, N.J., Cole, G.J., and Baier, H., Assembly of Lamina-Specific Neuronal Connections by Slit Bound to Type IV Collagen, 164-176, Copyright (2011) with permission from Elsevier. Full text @ Cell