ZFIN Image: Fujimoto et al., 2011, Fig. 1

Image

Figure Caption

Fig. 1

Examples of stable transgenic enhancer lines generated; ventral views, anterior to the top, double-immunohistochemistry for GFP and TH (green and red), confocal z-stacks. Scale bars are 50 μm. (A–C) Time-series of Tg(fezf2:egfp)^zc55 expression at 24 hpf, 48 hpf, and 72 hpf, shows non-overlap of TH and GFP expression. (D–D3) Tg(pitx3:egfp)^zc50 and (E–E3) Tg(f.TH.A:egfp)^zc56 at 72 hpf, shows partial overlap of enhancer expression and TH (arrows), but also wide spread expression of the enhancer in non-TH neurons (arrowheads). Insets in D2 and E2 show single confocal plane of double-labeling, emphasizing the minimal overlap of TH and GFP labeling.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 352(2), Fujimoto, E., Stevenson, T.J., Chien, C.B., and Bonkowsky, J.L., Identification of a Dopaminergic Enhancer Indicates Complexity in Vertebrate Dopamine Neuron Phenotype Specification, 393-404, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.