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Fig. s2
A-F are whole mount Shh ISH embryos at 10 som (A, D), 3 som (B, E) and bud (C, F) embryonic stage with expression noticed at midline in all stages. (A-C) is lateral view and, (D-F) is dorsal view. (G-H) are whole mount vegf ISH at 10 som. Inset in G shows the vegf expression in endoderm (e) adjoining the yolk and somite (s). I is immunostaining of 10 som section for VEGF protein. Yellow arrow indicates hypochord staining of VEGF protein.
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