ZFIN Image: Laux et al., 2011, Fig. s2

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Figure Caption

Fig. s2

Supporting Information Figure 2. Temporal relationship between pSmad1/5/8 activity and EGFP fluorescence in Tg(bre:egfp) embryos. Embryos were collected every 2 hr between 10–18 hpf and 28–38 hpf and assayed for pSmad1/5/8 via immunofluorescence (a–k), 100×; egfp mRNA via in situ hybridization (a2–k2), 80×; and EGFP fluorescence (a22–k22), 80×. Results demonstrate a 4-hr delay between pSmad1/5/8 expression and EGFP fluorescence, and an 8–10-hr perdurance of EGFP after pSmad1/5/8 is lost. Green indicates bre-driven EGFP expression, 80× magnification. White arrows, dorsal retina; yellow arrows, trigeminal ganglia. All images lateral view, anterior left.

Acknowledgments

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