PUBLICATION

Dynamic analysis of BMP-responsive smad activity in live zebrafish embryos

Authors
Laux, D.W., Febbo, J.A., and Roman, B.L.
ID
ZDB-PUB-110316-14
Date
2011
Source
Developmental dynamics : an official publication of the American Association of Anatomists   240(3): 682-694 (Journal)
Registered Authors
Roman, Beth
Keywords
zebrafish, bone morphogenetic proteins, Smads, transgenic reporter
MeSH Terms
  • Animals
  • Bone Morphogenetic Protein Receptors, Type I/genetics
  • Bone Morphogenetic Protein Receptors, Type I/metabolism
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Immunohistochemistry
  • In Situ Hybridization
  • Smad Proteins/genetics
  • Smad Proteins/metabolism*
  • Smad1 Protein/genetics
  • Smad1 Protein/metabolism
  • Smad5 Protein/genetics
  • Smad5 Protein/metabolism
  • Smad8 Protein/genetics
  • Smad8 Protein/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
21337466 Full text @ Dev. Dyn.
Abstract
Bone morphogenetic proteins (BMPs) are critical players in development and disease, regulating such diverse processes as dorsoventral patterning, palate formation, and ossification. These ligands are classically considered to signal via BMP receptor-specific Smad proteins 1, 5, and 8. To determine the spatiotemporal pattern of Smad1/5/8 activity and thus canonical BMP signaling in the developing zebrafish embryo, we generated a transgenic line expressing EGFP under the control of a BMP-responsive element. EGFP is expressed in many established BMP signaling domains and is responsive to alterations in BMP type I receptor activity and smad1 and smad5 expression. This transgenic Smad1/5/8 reporter line will be useful for determining ligand and receptor requirements for specific domains of BMP activity, as well as for genetic and pharmacological screens aimed at identifying enhancers or suppressors of canonical BMP signaling.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping