ZFIN Image: Madelaine et al., 2011, Fig. 2

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Figure Caption

Fig. 2 Activation of deltaA and deltaD by Ngn1 is rapid and cell autonomous.

Whole-mount in situ hybridization against deltaD (A–D) and deltaA (E–H) in embryos at 9 hpf (C, G) or 10 hpf (A, B, D, E, F, H). Tg(hs:ngn1) embryos heat-shocked at 7 hpf display ectopic expression of deltaD and deltaA 2 h after treatment (B, F). Similarly, mis-expression of Ngn1 at 8.5 hpf induces deltaD and deltaA expression in as little as 30 min (C, G). Single confocal sections of the neural plate at 10 hpf show that mosaic mis-expression of a Myc-tagged form of Ngn1 (in green) induces deltaD and deltaA (in red) in an apparently cell autonomous manner (D, H); nuclei are labelled with Topro (blue) to show that there are cells in the field of view that do not express Ngn1-myc or delta and that induced delta transcripts form a halo around the Ngn1-myc positive nucleus. Embryos are viewed dorsally with anterior up.

Acknowledgments

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Reprinted from Developmental Biology, 350(1), Madelaine, R., and Blader, P., A cluster of non-redundant Ngn1 binding sites is required for regulation of deltaA expression in zebrafish, 198-207, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.