Fig. S4

Fig. S4 Drug dose dependency of Axin1-YFP induction in myl7:TetA-GBD Cherry; TetRE:Axin1-YFP double transgenic embryos. (A) Dose–response to indicated doses of drugs applied at 48 h postfertilization (hpf). YFP RNA was detected by whole-mount in situ hybridization 4 h postinduction, EtOH control-treated embryos are shown at 24 h postinduction. (B) Classes of YFP expression in heart used for quantification. (C) Quantification of data shown in A. **P = 0.002 (Fisher exact test), ***P < 0.001 (χ² test), *P = 0.034 (χ² test). (D) Dex is limiting for induction in myl7:TetA-GBD Cherry; TetRE:Axin1-YFP double transgenic embryos. The indicated doses of drugs were applied at 48 hpf, YFP RNA was detected by whole-mount in situ hybridization 24 h postinduction. (E) Quantification of data shown in D). ***P < 0.001 (χ² test). (F) Dex is limiting for induction in TetRE:Axin1-YFP embryos injected with 90 pg TetA-GBD RNA. Embryos were treated with indicated doses of drugs at 7 hpf for 4.5 h. Axin1-YFP expression was detected by in situ hybridization and classified as in Fig S1. ***P < 0.001, *P = 0.019 (χ² test).